JRF offers wide range of in vitro assays that help us screen the candidates at a very early stage, by targeting certain important pharmacokinetic parameters and evaluating their specific roles in the body. It also helps to evaluate the critical ADMET parameters for pharmaceutical drug discovery.
Why in-vitro ADME?
Molecule to market is a complex process, often involving a series of diverse research investigations that ultimately help us select the best candidate for the eventual human use. A drug administered via the oral route undergoes various metabolic reactions inside the body, especially First Pass Metabolism in the liver. Certain drug candidates may be toxic at certain dose levels inside the body. Therefore, it becomes imperative to screen the best molecule(s) out of a library of compounds having a right proportion of therapeutic efficacy and pharmacokinetic property, thereby reducing time and cost involved in developing an effective drug for the target disease. In-vitro ADME models help us screen these candidates at a very early stage, by targeting certain important pharmacokinetic parameters and evaluating their specific roles in the body. While experiments such as Kinetic solubility evaluates simple physico-chemical properties of the drug like solubility, experiments like Cytochrome P450 inhibition can help us in deciphering the possible drug-drug and drug-food interactions that may affect the therapeutic efficacy of the molecule in the body.
In vitro ADME and PK
JRF Global conducts a complete range of various ADME assays, which are either essential for an integrated drug discovery program or as individual assays.
ASSAYS
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Parallel Artificial Membrane Permeability Assay (PAMPA)
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SGF (Simulated Gastric Fluid) and SIF (Simulated Intestinal Fluid) stability
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Microsomal Stability/Intrinsic Clearance
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Plasma Stability
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S9 Stability
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Plasma protein binding
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Reaction Phenotyping
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Blood to Plasma Ratio
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Brain Tissue Binding
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Cytochrome 450 Inhibition Assay
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CYP Inhibition by Cocktail Approach
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